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miniprotean iii apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad miniprotean iii apparatus
    Miniprotean Iii Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miniprotean+iii+apparatus/Mini-PROTEAN+Tetra+Cell+for+Ready+Gel+Precast+Gels/pmc03954548-122-17-17
    Average 90 stars, based on 1 article reviews
    miniprotean iii apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: ? M ? 2 -integrin-intercellular adhesion molecule-1 interactions drive the flow-dependent trafficking of Guillain-Barr? syndrome patient derived mononuclear leukocytes at the blood-nerve barrier in vitro
    Article Snippet: .. Equal amounts of protein (6 μg) were denatured, reduced and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10% slab gel) using the Bio-Rad MiniProtean III Apparatus (Hercules, CA), then electrotransfered to polyvinylidene fluoride membranes in transfer buffer (1:20 of 5X Towbin’s buffer [144g Glycine, 30.25g Tris, 2g SDS, made up to 1L with distilled water] + 10% methanol in distilled water) using the Bio-Rad Trans-Blot Apparatus (Hercules, CA), according to manufacturer instructions. ..

    Article Title: An immortalized human blood-nerve barrier endothelial cell line for in vitro permeability studies
    Article Snippet: .. Equal amounts of protein (15 μg) were denatured, reduced and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; 10% slab gel) using the Bio-Rad MiniProtean III Apparatus (Hercules, California, USA), then electrotransfered to polyvinylidene fluoride membranes in transfer buffer using the Bio-Rad Trans-Blot Apparatus, according to manufacturer instructions. ..

    Article Title: CCR2 Gene Deletion and Pharmacologic Blockade Ameliorate a Severe Murine Experimental Autoimmune Neuritis Model of Guillain-Barré Syndrome
    Article Snippet: .. Equal amounts of protein were denatured, reduced and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Bio-Rad MiniProtean III Apparatus, then electrotransfered to polyvinylidene fluoride membranes for western blot. ..

    SDS Page:

    Article Title: The Mitochondrial Small Heat Shock Protein HSP22 from Pea is a Thermosoluble Chaperone Prone to Co-Precipitate with Unfolding Client Proteins
    Article Snippet: .. Proteins were separated by SDS-PAGE according to standard protocols using polyacrylamide gels [10 to 13.5% ( w/v )], Tris-glycine/SDS buffer [ ], and a Miniprotean III apparatus (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility.
    Article Snippet: The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling).. In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats.. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples.

    Article Title: The secretion of the angiogenic and neurotrophic factor angiogenin is COPII and microtubule dependent.
    Article Snippet: The RNaseA superfamily member Angiogenin (ANG) is a secreted protein involved in neovascularization, cell proliferation and stress response.. Dysregulation of ANG expression is found in many cancers with poor prognosis and mutations in ANG are associated with neurodegenerative diseases.. While the uptake and nuclear translocation of ANG is relatively well characterised, little is known about how it reaches the plasma membrane and its mode of secretion.

    Electrophoresis:

    Article Title: Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility.
    Article Snippet: The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling).. In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats.. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples.

    Article Title: High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes.
    Article Snippet: Acute bouts of high-intensity intermittent exercise (HIIE) or sports are associated with changes in lymphocytes and platelet functions and we hypothesized that the purinergic system is involved with these alterations.. We investigated the activity of ectonucleotidases in platelets and lymphocytes as well as the platelet aggregation of futsal players in response to an acute protocol of HIIE.. Thus, 19 male semi-professional futsal players were submitted to 40 min of HIIE on a treadmill.

    Membrane:

    Article Title: Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility.
    Article Snippet: The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling).. In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats.. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples.

    Incubation:

    Article Title: Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility.
    Article Snippet: The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling).. In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats.. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples.

    Western Blot:

    Article Title: CCR2 Gene Deletion and Pharmacologic Blockade Ameliorate a Severe Murine Experimental Autoimmune Neuritis Model of Guillain-Barré Syndrome
    Article Snippet: .. Equal amounts of protein were denatured, reduced and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Bio-Rad MiniProtean III Apparatus, then electrotransfered to polyvinylidene fluoride membranes for western blot. ..



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